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    Lipid domain association of influenza virus proteins detected by dynamic fluorescence microscopy techniques (2013)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Veit, Michael (WE 5)
    Engel, Stephanie (WE 5)
    Thaa, Bastian (WE 5)
    Scolari, Silvia
    Herrmann, Andreas
    Quelle
    Cellular microbiology; 15(2) — S. 179–189
    ISSN: 1462-5822
    Sprache
    Englisch
    Verweise
    DOI: 10.1111/cmi.12045
    Pubmed: 23057766
    Kontakt
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    Tel. +49 30 838 51833 Fax. +49 30 838 451847
    email:viro@zedat.fu-berlin.de

    Abstract / Zusammenfassung

    Influenza virus is thought to assemble in raft domains of the plasma membrane, but many of the conclusions were based on (controversial) Triton extraction experiments. Here we review how sophisticated methods of fluorescence microscopy, such as FPALM, FRET and FRAP, contributed to our understanding of lipid domain association of the viral proteins HA and M2. The results are summarized in light of the current model for virus assembly and lipid domain organization. Finally, it is described how the signals that govern domain association in transfected cells affect replication of influenza virus.