Publikationsdatenbank

Lipid domain association of influenza virus proteins detected by dynamic fluorescence microscopy techniques (2013)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Veit, Michael (WE 5)

Engel, Stephanie (WE 5)

Thaa, Bastian (WE 5)

Scolari, Silvia

Herrmann, Andreas

Quelle

Cellular microbiology

Bandzählung: 15

Heftzählung: 2

Seiten: 179 – 189

ISSN: 1462-5822

Sprache

Englisch

Verweise

DOI: 10.1111/cmi.12045

Pubmed: 23057766

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Influenza virus is thought to assemble in raft domains of the plasma membrane, but many of the conclusions were based on (controversial) Triton extraction experiments. Here we review how sophisticated methods of fluorescence microscopy, such as FPALM, FRET and FRAP, contributed to our understanding of lipid domain association of the viral proteins HA and M2. The results are summarized in light of the current model for virus assembly and lipid domain organization. Finally, it is described how the signals that govern domain association in transfected cells affect replication of influenza virus.