Publication Database

Signal peptide cleavage from GP3 enabled by removal of adjacent glycosylation sites does not impair replication of equine arteritis virus in cell culture, but the hydrophobic C-terminus is essential (2014)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Matczuk, Anna Karolina (WE 5)

Veit, Michael (WE 5)

Quelle

Virus research : an international journal of molecular and cellular virology

Bandzählung: 183

Seiten: 107 – 111

ISSN: 0168-1702

Sprache

Englisch

Verweise

DOI: 10.1016/j.virusres.2014.02.005

Pubmed: 24556360

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. Surprisingly, the infectivity of EAV containing GP3 with cleaved signal peptide was not impaired and GP3 with cleaved signal peptide associates with GP2/4 in virus particles. In contrast, viruses containing GP3 with deleted hydrophobic C-terminus rapidly reverted back to wild type. The data support our model that the signal peptide is exposed to the lumen of the ER and the C-terminus peripherally attaches GP3 to membranes.