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    Characterisation of the beta-tubulin gene of Cylicocyclus nassatus? (1999)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Pape, M
    von Samson-Himmelstjerna, G (WE 13)
    Schnieder, T
    Quelle
    International Journal for Parasitology; 29(12) — S. 1941–1947
    ISSN: 0020-7519
    Sprache
    Englisch
    Verweise
    Pubmed: 10961850
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    Tel.+49 30 838 62310 Fax.+49 30 838 62323
    email:parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    mRNA and genomic DNA were isolated from adult Cylicocyclus nassatus, and the mRNA was reverse transcribed. The cDNA was PCR amplified using degenerate primers designed according to the alignment of the beta-tubulin amino acid sequences of other species. To complete the coding sequence, the 3' end was amplified with the 3' RACE, and for amplification of the 5' end the SL1-primer was used. The cDNA of the beta-tubulin gene of C. nassatus spans 1429 bp and encodes a protein of 448 amino acids. Specific primers were developed from the cDNA sequence to amplify the genomic DNA sequence and to analyse the genomic organisation of the beta-tubulin gene. The complete sequence of the genomic DNA of the beta-tubulin gene of C. nassatus has a size of 2652 bp and is organised into nine exons and eight introns. The identities with the exons of the gru-1 beta-tubulin gene of Haemonchus contortus range between 79% and 97%.