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    Improved in vitro cultivation of larvae of the bovine lungworm Dictyocaulus viviparus (1998)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    von Samson-Himmelstjerna, G (WE 13)
    Rickling, S
    Schnieder, T
    Quelle
    Parasitology research; 84(8) — S. 682–684
    ISSN: 0932-0113
    Sprache
    Englisch
    Verweise
    Pubmed: 9747942
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    +49 30 838 62310
    parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Parasitic larvae of Dictyocaulus viviparus are of major importance for the development of immunity in cattle. The conditions for in vitro cultivation of D. viviparus larvae as well as their morphology have thus far been only poorly investigated. Exsheathed larvae were cultivated in vitro in RPMI-1640 (Gibco-BRL, pH 7.2) containing 20% newborn Calf serum, 200 U Moronal/ml, and 200 U penicillin/streptomycin/ml. Incubation was performed at 39.5 degrees C at 0, 5%, 10%, and 20% CO2. Average development rates to third-moult (3 M) or fourth-stage (L4) larvae at 5% CO2 incubation were 8.33% (SD +/- 7.76%), 22.52% (SD +/- 13.09%) at 10% CO2, and 38.01% (SD +/- 15.63%) at 20% CO2. These differences were statistically significant. Some morphological features of these larvae are described.