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    Species differentiation of lungworms (Dictyocaulidae) by polymerase chain reaction/restriction-fragment-length polymorphism of second internal transcribed spacers of ribosomal DNA (1996)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Schnieder, T
    Epe, C
    von Samson-Himmelstjerna, G (WE 13)
    Quelle
    Parasitology research; 82(5) — S. 392–394
    ISSN: 0932-0113
    Sprache
    Englisch
    Verweise
    Pubmed: 8738276
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    +49 30 838 62310
    parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The bovine lungworm Dictyocaulus viviparus is found in cattle and deer species (roe deer, fallow deer). Although a separate lungworm species (D. eckerti) from roe deer has been described, this description, based on minor morphological differences, is not generally accepted. The aim of the present study was to examine genetic differences among four lungworm species isolated from cattle, sheep, horses, and fallow deer. Ribosomal second internal transcribed spacers (ITS2) of individual worms of all species were amplified by polymerase chain reaction (PCR), and restriction-fragment-length polymorphisms were analyzed after digestion with five different restriction enzymes. After electrophoretic separation of the digested PCR products, all four lungworm species showed different banding patterns. Lungworms from cattle (D. viviparus) and those from fallow deer (D. eckerti) were genetically distinct. These results are consistent with the hypothesis that the taxonomic classification of D. eckerti as a separate species is correct.