Publication Database

Pathotyping bla CTX-M Escherichia coli from Nigeria (2013)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Olowe, Olugbenga Adekunle

Choudhary, Suman

Schierack, Peter

Wieler, Lothar H (WE 7)

Makanjuola, Olufunmilola Bamidele

Olayemi, Albert B

Anjum, Muna

Quelle

European journal of microbiology & immunology

Bandzählung: 3

Heftzählung: 2

Seiten: 120 – 125

ISSN: 2062-509x

Sprache

Englisch

Verweise

DOI: 10.1556/EuJMI.3.2013.2.5

Pubmed: 24265928

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated.

To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine bla CTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes.

Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles.

The findings describe a broadly disseminated, bla CTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority.