Fachbereich Veterinärmedizin



    Pathotyping bla CTX-M Escherichia coli from Nigeria (2013)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Olowe, Olugbenga Adekunle
    Choudhary, Suman
    Schierack, Peter
    Wieler, Lothar H (WE 7)
    Makanjuola, Olufunmilola Bamidele
    Olayemi, Albert B
    Anjum, Muna
    European journal of microbiology & immunology; 3(2) — S. 120–125
    ISSN: 2062-509x
    DOI: 10.1556/EuJMI.3.2013.2.5
    Pubmed: 24265928
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843

    Abstract / Zusammenfassung

    Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated.

    To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine bla CTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes.

    Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles.

    The findings describe a broadly disseminated, bla CTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority.