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    Method for the preparation of mucosal flaps from the jejunum of laying hens for transporter studies in Ussing chambers (2013)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ruhnke, Isabelle (WE 4)
    Röhe, Ilen (WE 4)
    Meyer, Wilfried
    Kröger, Susan (WE 4)
    Neumann, Konrad
    Zentek, Jürgen (WE 4)
    Quelle
    Archives of animal nutrition; 67(2) — S. 161–168
    ISSN: 1745-039x
    Sprache
    Englisch
    Verweise
    DOI: 10.1080/1745039X.2013.776328
    Pubmed: 23521695
    Kontakt
    Institut für Tierernährung

    Königin-Luise-Str. 49
    Gebäude 8
    14195 Berlin
    Tel.+49 30 838 52256 Fax.+49 30 838-55938
    email:tierernaehrung@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Ussing chambers are frequently used for in vitro evaluation of intestinal transport physiology. The current study describes investigating the jejunal tissue from laying hens using a specific preparation method and evaluates the effect of glutamine in the maintenance buffer. Tunica mucosa was stripped from 104 jejunal samples from 10 hens and stabilised by a net device. Fifty samples were maintained with modified Krebs-Henseleit buffer (Control), 54 samples with additional 5 mM glutamine (Group Gln). The percentage of responding samples varied between 87 and 100%. Mean short circuit current (ΔI sc,) [µA/cm(2)] of samples exposed to 10 mM glucose in the Control group and Group Gln was 17.0 and 14.6 (p = 0.836), respectively, of samples exposed to 100 µM phloridzin -13.3 and -11.8 (p = 0.712), respectively, and of samples exposed to 100 µM carbachol 4.7 and 3.7 (p = 0.450), respectively. In conclusion, the net-supported method enabled a reliable investigation of jejunum from laying hens. Glutamine in the maintenance buffer was of no significant benefit.