Publication Database

Histological evaluation of the sheep's cervical spine segment 3/4--a histomorphological and histomorphometrical study (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Koch, C

Kandziora, F

Huenigen, H (WE 1)

Plendl, J (WE 1)

Quelle

Anatomia, histologia, embryologia

Bandzählung: 37

Heftzählung: 3

Seiten: 200 – 4

ISSN: 0340-2096

Sprache

Englisch

Verweise

Pubmed: 18162094

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The 2-year-old sheep is a frequently used model in spinal orthopaedic research; however, so far no investigations have been performed on physiological tissue of untreated animals. In this study, the cervical spine segment 3/4 of eight 2-year-old female Merino sheep was evaluated histologically and histomorphometrically. Specimens were stained with Masson-Goldner-Trichrome, Safranin-Orange/Lightgreen, Safranin-Orange/von Kossa and Astrablue. Analysis of bone mass, mass of cartilage and mineralized cartilage in a defined region of interest was performed. A new finding of the study was that--although 2-year-old sheep is regarded as adult--tissue of the growth plate was still detected. Moreover, this study revealed that in the nucleus pulposus of the 2-year-old sheep notochordal cells are still existent, suggesting a complex remodelling of the nucleus pulposus in sheep.