Publikationsdatenbank

Einfluß verschiedener Zucker und Zuckeraustauschstoffe auf die Bakterienflora und deren Enzymaktivitäten im Caecum der Ratte nach Gewöhnung (1989)

Art

Hochschulschrift

Autor

Hähnel, Anita

Quelle

Berlin, 1989 — 103 Seiten

Kontakt

Institut für Tierschutz, Tierverhalten und Versuchstierkunde

Königsweg 67
14163 Berlin
+49 30 838 61146
tierschutz@vetmed.fu-berlin.de

Abstract / Zusammenfassung

7 groups of male Wistar rats were prefed for twelve days with different sugars and sugar substitutes. The influence on the bacterial flora of the caecum and on its enzyme equipment should be investigated.There was a clearly visible germ-selection and change in germ number between rats prefed with sugar substitutes and control animals; gram positive germs disappeared nearly all when the rats were prefed with sugar substitutes except in rats prefed with Maltit.The average number of germs was 1000000000000-10000000000000/caecum. There was a significant difference in germ numbers in the caeca of the control animals and those prefed with sugar substitutes. The rats fed with glucose showed the lowest germ number.Concerning the activity of glycosidases which were the most important enzymes, in this investigation, several differences between control animals and those fed with sugar substitutes could be described. The a-galactosidase was never detectable in control animals and in maltit-fed rats. In contrast, ß-galactosidase couldn"t be seen only in the control animals. The most intense colour reaction of the test kit was induced by ß-glucuronidases of rats prefed with Palatinit®; the control animals showed the slightest reaction. a- and ß-glucosidase could be detected with small deviations in all groups. An exception was the ß-N-acetyl-glucosaminidase which was only found with low intensity in rats prefedwith Palatinit®, Polydextrose® and sorbitol.The selection of some germs and increasing in total number of germs including the changed enzyme activities will be regarded as a sign of the metabolic adaptation to the changed supply of substrate in the caecum without pathological consequences to the host organism.