Gebäude 21, 1. OG
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The problems of over-feeding which civilisation causes, and of nutritional mal-functionssuch as diabetes, make it necessary to search for food additives with a low nutritional valuein comparison with conventional foods, meaning low usable energy-ingredients which give agood feeling of satiety (by providing a good quantity of bulkage) and a sufficiently pleasingflavour.Four test substances were examined: methoxyinulin, carboxyinulin, sorbitol and xylitol.Each of the test substances was examined on one group of conventional and one group ofgermfree rats. Five rats were included in each control group, on which cellulose wasexamined. Six animals were selected on the randomisation principle for each of the otherexperimental groups.Radioactively labeled test substances were administered orally to all the animals in definedquantities. After preset period of time (30 hours after administration in the case of cellulose,after which time only insignificant further changes occur and 48 hours for all othersubstances) distribution examinations were carried out after sacrifice using Nembutal©(pentobarbital-sodium). The animals exhalations were monitored continuously forradioactivity throughout the experiment. All quantities discovered were balanced out anddivided into an unassimilated, eliminated proportion (in the faces) and a proportion which hadbeen assimilated as energy(14 CO 2 in the exhaled air). Thus the actual energy- utilisation inrelation to the caloric value could be determined.It proved possible to demonstrate tha tthere was a great difference in the energy metabolismof the various materials under examination. The reliability of the results is confirmed by thegood level of comparability of the cellulose results, which were obtained under the sameexperimental conditions as the other four substances, with he experimental results reportedby other authors.Although xylitol showed the greatest energy availability at 33.65% energy utilisation, scoredonly 39.04% and was thus behind sorbitol with 43.94%. Methoxyinulin was used as energyin the rate of 24.03%, followed by the control substance cellulose with 18.63%. The lowestvalue for energy- usage was obtained with carboxyinulin with only 12.46%.Using radioactively labeled substances and the disposition method, it was possible tomeasure the energy efficiency of each substance in comparison with cellulose. The energyutilisation was demonstrated to be perceptibly higher in three of the substances (sorbitol,xylitol and methoxyinulin) and significantly lower in one of them (carboxyinulin).