Fachbereich Veterinärmedizin



    Technical note:
    evaluation of odor from vaginal discharge of cows in the first 10 days after calving by olfactory cognition and an electronic device (2013)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Sannmann, I (WE 19)
    Burfeind, O (WE 19)
    Suthar, V (WE 19)
    Bos, A
    Bruins, M
    Heuwieser, W (WE 19)
    Journal of Dairy Science; 96(9) — S. 5773–5779
    ISSN: 0022-0302
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000019650
    DOI: 10.3168/jds.2013-6813
    Pubmed: 23810587
    Tierklinik für Fortpflanzung

    Königsweg 65
    Haus 27
    14163 Berlin
    Tel.+49 30 838 62618 Fax.+49 30 838 62620

    Abstract / Zusammenfassung

    The objective of this study was to determine test characteristics (i.e., intra- and interobserver variability, intraassay variability, sensitivity, and specificity) of an evaluation of odor from vaginal discharge (VD) of cows in the first 10 d postpartum conducted by olfactory cognition and an electronic device, respectively. In experiment 1, 16 investigators (9 veterinary students and 7 licensed veterinarians) evaluated 5 VD samples each on 10 different days. The kappa test revealed an agreement between investigators (interobserver) of κ=0.43 with a Fleiss adjusted standard error of 0.0061. The overall agreement was the same for students (κ=0.28) and veterinarians (κ=0.28). Mean agreement within observers (intraobserver) was κ=0.52 for all observers, and 0.49 and 0.62 for students and veterinarians, respectively. In experiment 2, the repeatability of an electronic device (DiagNose; C-it, Zutphen, the Netherlands) was tested. Therefore, 5 samples of VD from 5 cows were evaluated 10 times each. The repeatability was 0.97, determined by Cronbach's α. In experiment 3, 20 samples collected from healthy cows and 20 of cows with acute puerperal metritis were evaluated by the 16 investigators and the DiagNose using a dichotomous scale (1=cow with acute puerperal metritis; 0=healthy cow). Sensitivity and specificity of olfactory evaluation was 75.0 and 60.1% compared with 92.0 and 100%, respectively, for the electronic nose device. The study revealed a considerable subjectivity of the human nose concerning the classification into healthy and sick animals based on the assessment of vaginal discharge. The repeatability of the electronic nose was higher. In conclusion, the DiagNose system, although imperfect, is a reasonable tool to improve odor assessment of VD. The current system, however, is not suitable as a screening tool in the field. Further research is warranted to adapt such electronic devices to practical on-farm screening tools.