Publikationsdatenbank

Extra domain B fibronectin as a target for near-infrared fluorescence imaging of rheumatoid arthritis affected joints in vivo (2009)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Vollmer, Sonja

Vater, Axel

Licha, Kai

Gemeinhardt, Ines

Gemeinhardt, Ole

Voigt, Jan

Ebert, Bernd

Schnorr, Jörg

Taupitz, Matthias

Macdonald, Rainer

Schirner, Michael

Quelle

Molecular imaging : official journal of the Society for Molecular Imaging

Bandzählung: 8

Heftzählung: 6

Seiten: 330 – 40

ISSN: 1536-0121

Sprache

Englisch

Verweise

Pubmed: 20003891

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

We investigated a molecular imaging approach for the detection of collagen-induced arthritis in rats by targeting the extra domain B (ED-B) of the extracellular matrix protein fibronectin. ED-B is a highly conserved domain (identical in human and rats) that is produced by alternative splicing during embryonic development and during vascular remodeling such as angiogenesis. The hallmark of rheumatoid arthritis is synovitis leading to both angiogenesis in the synovium and the promotion of cartilage and bone disruption. For in vivo diagnostics, the ED-B-binding single-chain antibody fragment AP39 was used as a targeting probe. It was covalently linked to the near-infrared dye tetrasulfocyanine (TSC) to be visualized by near-infrared fluorescence imaging. The resulting AP39-TSC conjugate was intravenously administered to rats with collagen-induced arthritis and the respective controls. Ovalbumin-TSC was used as control conjugate. Optical imaging over a time period of 24 hours using a planar imaging setup resulted in a clear enhancement of fluorescence intensity in joints with moderate to severe arthritis compared with control joints between 3 and 8 hours postinjection. Given that AP39 is a fully human antibody fragment, this molecular imaging approach for arthritis detection might be translated to humans.