Fachbereich Veterinärmedizin



    Cytokine induction and virulence mechanisms of Arcobacter butzleri in human macrophages (2013)

    zur Brügge, Jennifer
    Gölz, Greta (WE 8)
    Tedin, Karsten (WE 7)
    Einspanier, Ralf (WE 3)
    Alter, Thomas (WE 8)
    Sharbati, Soroush (WE 3)
    17th International Workshop on Campylobacter, Helicobacter and Related Organisms, CHRO 2013
    Aberdeen, UK, 15. – 19.09.2013
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    +49 30 838 62550

    Abstract / Zusammenfassung

    Arcobacter butzleri ( A. burzleri ) is a member of the family Campylobacleraceae and is considered an emerging zoonotic pathogen. Although no obvious effects on animal health has been reported, severe gastroenteritis can occur in humans e.g. via consumption of contaminated food, mainly pork and poultry. However, the molecular mechanisms of the human innate immune response to A. butzleri remains poorly understood. To evaluate the influence of A. butzleri on human macrophages, three different strains (human as well as chicken isolates) were studied in an in vitro infection model using macrophages derived from the monocitic cell line THP-1. Custom RT-qPCR arrays were used to investigate mRNA expression of relevant cytokines typically produced in macrophages during infection. The expression of IL-1a, IL-1b, IL-6, IL-8, IL-12b and TNF was highly induced by all strains while IL-12a and iNOS remained unaffected. Survival assays revealed the ability of the pathogen to survive and resist the hostile phagosomal environment of phagocytic immune cells. Current attempts are directed towards the ability of A. butzleri to induce apoptosis in macrophages. In this context , the potential role of IL-lb triggering the Caspase 8 pathway is examined. The current study will help to gain insight into the molecular mechanisms of host-interaction with this emerging zoonotic organism enabling the generation of future strategies targeting A.butzleri induced diseases.