Fachbereich Veterinärmedizin



    Distribution of virulence genes, adhesion and invasion of Arcobacter butzleri (2013)

    Karadas, Gül (WE 8)
    Alter, Thomas (WE 8)
    Sharbati, Soroush (WE 3)
    Bücker, Roland
    Messelhäußer, Ute
    Gölz, Greta (WE 8)
    17th International Workshop on Campylobacter, Helicobacter and Related Organisms (CHRO)
    Aberdeen, UK, 15. – 19.09.2013
    URL (Volltext): http://www.vetmed.fu-berlin.de/einrichtungen/institute/we08/poster/2013_karadas/index.html
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
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    Abstract / Zusammenfassung

    The pathogenic potential of Arcobacter butzleri isolates was investigated by detecting the presence of putative virulence genes and analysing the adhesive and invasive capabilities in cell cultures of human cell lines. The presence of 10 putative virulence genes in 52 A. butzleri isolates was determined by PCR. The genes (ciaB, mviN, pldA, tlyA, cj1349 and cadF were detected in all, while irgA (15 %), iroE (60 %), hecB (44 %) and hecA (13 %) were detected only in few A. butzleri isolates. On HT-29 cells, four out of six isolates adhered and three of them were able to invade, while all six isolates adhered and invaded Caco-2 cells with higher degrees. The genes ciaB, cadF and cj1349 of all six isolates were sequenced but no considerable changes of the amino acids in putative functional domains were observed. We conclude that selected A. butzleri isolates adhere to and invade various human cell lines, which emphasize their human pathogenic potential. The efficiency of invasion depends on the eukaryotic cell line and the individual bacterial strain used. We could not show any functional correlation between the amino acid sequence of CadF, CiaB or Cj1349 and the adhesive or invasive phenotype.