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This experiment studied the effects of 0.2% inclusion of a slow release formulation based on a mixture of flavoring compounds with benzoic acid as the main ingredient (Avimatrix®, Novus Int) in pelleted feed on broiler performance under Salmonella-challenged conditions. The ileal microflora was also evaluated.
No antibiotics were used in the trial. Ten replicates, each with two birds, were used per treatment. Each bird was inoculated orally with 1 x 106 Cfu/ml of Salmonella enteritidis at day 10. The test birds showed a significant improvement in feed conversion when compared to the control group (p<0.001). Additional studies analyzed the microflora of the ileal digesta from 8 birds per treatment using Quantitative Real Time PCR. The embedded benzoic acid in the diet significantly increased the total Lactobacillus count (p<0.05), in particular lactobacilli strains with known probiotic effect, and the ratio of Lactobacilli sp / E. coli. These data suggests that the tested product influences the microflora balance. A more stable gut flora could explain the improved performance in Salmonella-challenged birds in the test group when compared to the untreated group.