Fachbereich Veterinärmedizin



    Biofilm building capacity of Salmonella enterica strains from the poultry farm environment (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Schonewille, Esther
    Nesse, Live L
    Hauck, Rüdiger (WE 15)
    Windhorst, Daniel
    Hafez, Hafez M (WE 15)
    Vestby, Lene K
    FEMS immunology and medical microbiology; 65(2) — S. 360–365
    ISSN: 0928-8244
    DOI: 10.1111/j.1574-695X.2012.00966.x
    Pubmed: 22448648
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    Tel.+49 30 838 62676 Fax.+49 30 838 62690

    Abstract / Zusammenfassung

    The Biofilm (BF) building capacity of different serotypes of Salmonella enterica derived from the poultry farm environment was investigated. Starting point for the investigation was the question if farm-isolated Salmonella serotypes with high importance for poultry meat and egg production are capable of forming a BF under defined laboratory conditions. Several isolates from different stages of the production cycle were chosen and compared to laboratory grown strains of the same serotype. BF building capacity was analyzed in a 96-well format during a time period of 2 days. Pulse field gel electrophoresis was used to establish a relationship between different isolates. The BF building capacity of a monospecies BF was strongly dependent on the temperature used for incubation. Results indicated further that certain farm isolates were capable of forming BF under laboratory conditions, whereas laboratory grown strains were not. Considerable differences between different field serovars and within one serovar exist. In conclusion, the BF building capacity of poultry-derived isolates is a function of adaptation to their host environment. Thus, the control of BF as a reservoir for Salmonella in the farm environment is of crucial importance for the overall improvement of food safety. Mechanical and substance-based approaches for this control exist in several variations, but overall decontamination success is difficult to achieve and needs to be especially adapted to the farm environment.