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    Elucidation of colonization time and prevalence of thermophilic Campylobacter species during turkey rearing using multiplex polymerase chain reaction (2012)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    El-Adawy, H
    Hotzel, H
    Tomaso, H
    Neubauer, H
    Hafez, H M (WE 15)
    Quelle
    Poultry Science; 91(2) — S. 454–459
    ISSN: 0032-5791
    Sprache
    Englisch
    Verweise
    DOI: 10.3382/ps.2010-01810
    Pubmed: 22252360
    Kontakt
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    Tel.+49 30 838 62676 Fax.+49 30 838 62690
    email:gefluegelkrankheiten@vetmed.fu-berlin

    Abstract / Zusammenfassung

    Two turkey flocks (male and female) and the environment of their house were investigated for the presence of thermophilic Campylobacter. Sample DNA was extracted directly from fecal material and environmental samples. Bacterial identification was done using a modified Campylobacter species specific multiplex PCR. The times needed for colonization and prevalence in male and female turkeys were determined independently. All environmental samples collected before restocking were negative in the PCR analysis, showing a good hygiene and biosecurity system. The first positive PCR results were obtained in drinking water samples at 6 d of age. Colonization occurred between the second and third week of age, starting in female birds and then followed by the males. Campylobacter jejuni was detected by multiplex PCR at first; later on, Campylobacter coli and mixtures of both were seen. After the 9 wk of age, the colonization of the flocks was completed. Great attention should be given to drinking water as a supposed source of Campylobacter contamination. Multiplex PCR proved to be a rapid, sensitive, and cheap tool for the diagnosis of Campylobacter contamination.