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    Simian varicella virus open reading frame 63/70 expression is required for efficient virus replication in culture (2011)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Brazeau, Elizabeth
    Wellish, Mary
    Kaufer, Benedict B (WE 5)
    Tischer, B Karsten (WE 5)
    Gray, Wayne
    Zhou, Fuchun
    Osterrieder, Nikolaus (WE 5)
    Hanlon, Teri
    Golive, Anjani
    Hall, Travis
    Nair, Sreekala
    Owens, Gregory P
    Mueller, Niklaus H
    Cohrs, Randall J
    Pugazhenthi, Subbiah
    Gilden, Don
    Mahalingam, Ravi
    Quelle
    Journal of neurovirology; 17(3) — S. 274–280
    ISSN: 1355-0284
    Sprache
    Englisch
    Verweise
    DOI: 10.1007/s13365-011-0025-6
    Pubmed: 21479719
    Kontakt
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    Tel. +49 30 838 51833 Fax. +49 30 838 451847
    email:viro@zedat.fu-berlin.de

    Abstract / Zusammenfassung

    Simian varicella virus (SVV) open reading frame (ORF) 63, duplicated in the virus genome as ORF 70, is homologous to varicella zoster virus ORF 63/70. Transfection of bacterial artificial chromosome clones containing the wild-type SVV genome and mutants with stop codons in ORF 70, in both ORFs 63 and 70 and the repaired virus DNA sequences into Vero cells produced a cytopathic effect (CPE). The onset of CPE was much slower with the double-mutant transfectants (10 days vs. 3 days) and plaques were smaller. While SVV ORF 63 is not required for replication in culture, its expression leads to robust virus replication.