Publication Database

Simian varicella virus open reading frame 63/70 expression is required for efficient virus replication in culture (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Brazeau, Elizabeth

Wellish, Mary

Kaufer, Benedict B (WE 5)

Tischer, B Karsten (WE 5)

Gray, Wayne

Zhou, Fuchun

Osterrieder, Nikolaus (WE 5)

Hanlon, Teri

Golive, Anjani

Hall, Travis

Nair, Sreekala

Owens, Gregory P

Mueller, Niklaus H

Cohrs, Randall J

Pugazhenthi, Subbiah

Gilden, Don

Mahalingam, Ravi

Quelle

Journal of neurovirology

Bandzählung: 17

Heftzählung: 3

Seiten: 274 – 280

ISSN: 1355-0284

Sprache

Englisch

Verweise

DOI: 10.1007/s13365-011-0025-6

Pubmed: 21479719

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Simian varicella virus (SVV) open reading frame (ORF) 63, duplicated in the virus genome as ORF 70, is homologous to varicella zoster virus ORF 63/70. Transfection of bacterial artificial chromosome clones containing the wild-type SVV genome and mutants with stop codons in ORF 70, in both ORFs 63 and 70 and the repaired virus DNA sequences into Vero cells produced a cytopathic effect (CPE). The onset of CPE was much slower with the double-mutant transfectants (10 days vs. 3 days) and plaques were smaller. While SVV ORF 63 is not required for replication in culture, its expression leads to robust virus replication.