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    Hd86, the Bm86 tick protein ortholog in Hyalomma scupense (syn. H. detritum):
    expression in Pichia pastoris and analysis of nucleotides and amino acids sequences variations prior to vaccination trials (2012)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ben Said, Mourad
    Galai, Yousr
    Canales, Mario
    Nijhof, Ard Menzo (WE 13)
    Mhadhbi, Moez
    Jedidi, Mohamed
    de la Fuente, José
    Darghouth, Mohamed Aziz
    Quelle
    Veterinary Parasitology; 183(3/4) — S. 215–223
    ISSN: 0304-4017
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.vetpar.2011.07.049
    Pubmed: 21871736
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    +49 30 838 62310
    parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The genus Hyalomma includes the most frequent tick species infesting livestock in North Africa, one of these species, Hyalomma scupense (syn. H. detritum) is particularly important due to its role in the transmission of tropical theileriosis to cattle (Theileria annulata infection). We have cloned and characterized the orthologs of the Bm86 gene from H. scupense strains collected over Tunisia in 2006 and 2009. The recombinant protein rHd86 was expressed in Pichia pastoris for vaccination purpose using a transcript from the 2006 strain. The rHd86 was then purified from the yeast culture supernatant by a filtration and a size exclusion process. It was recognized by specific anti-Bm86 antisera. An important extent of inter-specific diversity ranging from 35 to 40% was recorded between Hd86 and Bm86/Bm95 proteins whilst a very limited level of intra-specific diversity (1.7%) occurred between the Hd86 vaccine candidate protein and its homologues from H. scupense strains collected in 2009. These results emphasise the need for assessing the efficacy against H. scupense and others important cattle Hyalomma species in Tunisia of our Hd86 vaccine candidate alongside with a Bm86 vaccine.