Fachbereich Veterinärmedizin



    An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Ma, Guanggang (WE 5)
    Eschbaumer, Michael
    Said, Abdelrahman (WE 5)
    Hoffmann, Bernd
    Beer, Martin
    Osterrieder, Nikolaus (WE 5)
    PLoS one; 7(4) — S. e34425
    ISSN: 1932-6203
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000015315
    DOI: 10.1371/journal.pone.0034425
    Pubmed: 22511939
    Institut für Virologie

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    Abstract / Zusammenfassung

    Bluetongue virus (BTV) can infect most species of domestic and wild ruminants causing substantial morbidity and mortality and, consequently, high economic losses. In 2006, an epizootic of BTV serotype 8 (BTV-8) started in northern Europe that caused significant disease in cattle and sheep before comprehensive vaccination was introduced two years later. Here, we evaluate the potential of equine herpesvirus type 1 (EHV-1), an alphaherpesvirus, as a novel vectored DIVA (differentiating infected from vaccinated animals) vaccine expressing VP2 of BTV-8 alone or in combination with VP5. The EHV-1 recombinant viruses stably expressed the transgenes and grew with kinetics that were identical to those of parental virus in vitro. After immunization of mice, a BTV-8-specific neutralizing antibody response was elicited. In a challenge experiment using a lethal dose of BTV-8, 100% of interferon-receptor-deficient (IFNAR(-/-)) mice vaccinated with the recombinant EHV-1 carrying both VP2 and VP5, but not VP2 alone, survived. VP7 was not included in the vectored vaccines and was successfully used as a DIVA marker. In summary, we show that EHV-1 expressing BTV-8 VP2 and VP5 is capable of eliciting a protective immune response that is distinguishable from that after infection and as such may be an alternative for BTV vaccination strategies in which DIVA compatibility is of importance.