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    Tissue and cellular localization of nanoparticles using 35S labeling and light microscopic autoradiography (2013)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Holzhausen, Cornelia (WE 12)
    Gröger, Dominic
    Mundhenk, Lars (WE 12)
    Welker, Pia
    Haag, Rainer
    Gruber, Achim D (WE 12)
    Quelle
    Nanomedicine : nanotechnology, biology, and medicine; 9(4) — S. 465–468
    ISSN: 1549-9642
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.nano.2013.02.003
    Pubmed: 23434676
    Kontakt
    Institut für Tierpathologie

    Robert-von-Ostertag-Str. 15
    Gebäude 12
    14163 Berlin
    +49 30 838 62450

    Abstract / Zusammenfassung

    Microscopical visualization of nanoparticles in tissues is essential for assessing their distribution in whole organisms and their interaction with the cellular microenvironment, including possible toxic effects. However, labeling of nanoparticles with fluorescent dyes may affect their physicochemical properties. Moreover, the detection of organic nanoparticles in their tissue context often poses a particular challenge due to their closer similarities with biomolecules. As part of a biodistribution and toxicity study on organic anti-inflammatory nanoscaled dendritic polyglycerol sulfate amine (dPGS amine) we have established light microscopic autoradiography (LMA) for the tracking of (35)S labeled dPGS in standard histopathological tissue samples following intravenous injection in mice. The dPG(35)S amine was specifically localized in hepatic Kupffer cells with no histopathologic evidence of toxic, degenerate or inflammatory side effects. The combination of radiolabeling of organic nanoparticles with LMA offers a novel approach for their localization in microscopical slides, also allowing for a simultaneous standard toxicopathology analysis.

    In this study, a novel light microscopic autoradiography utilizing (35)S isotope demonstrates a combined approach to visualize nanoparticle locations in microscopic slides with no obvious toxicity to the studied cells and with minimal external hazard.