Fachbereich Veterinärmedizin



    The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Azab, Walid (WE 5)
    Zajic, Lara (WE 5)
    Osterrieder, Nikolaus (WE 5)
    Veterinary Research; 43(1) — S. 61
    ISSN: 0928-4249
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000015307
    DOI: 10.1186/1297-9716-43-61
    Pubmed: 22909178
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51833

    Abstract / Zusammenfassung

    Equine herpesvirus type 1 and 4 (EHV-1 and EHV-4) glycoprotein H (gH) has been hypothesized to play a role in direct fusion of the virus envelope with cellular membranes. To investigate gH's role in infection, an EHV-1 mutant lacking gH was created and the gH genes were exchanged between EHV-1 and EHV-4 to determine if gH affects cellular entry and/or host range. In addition, a serine-aspartic acid-isoleucine (SDI) integrin-binding motif present in EHV-1 gH was mutated as it was presumed important in cell entry mediated by binding to α4β1 or α4β7 integrins. We here document that gH is essential for EHV-1 replication, plays a role in cell-to-cell spread and significantly affects plaque size and growth kinetics. Moreover, we could show that α4β1 and α4β7 integrins are not essential for viral entry of EHV-1 and EHV-4, and that viral entry is not affected in equine cells when the integrins are inaccessible.