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    New Developments in Diagnostic Tools-opportunities for Food Safety and Outbreak Investigations (2013)

    Art
    Vortrag
    Autor
    Alter, Thomas (WE 8)
    Forschungsprojekt
    Entwicklung und Validierung innovativer produktionsintegrierter mikrobiologischer Stufenkontrollsysteme in der Fleischerzeugung zur Reduktion von Campylobacter (Teilprojekt 4 im Verbundprojekt InnoStep)
    Kongress
    10th Year Anniversary of Veterinary Public Health Centre for Asia Pacific
    The Imperial Mae Ping Hotel, Chiang Mai, Thailand, 02. – 06.07.2013
    Quelle
    10th Year Anniversary of Veterinary Public Health Centre for Asia Pacific
    Chiang Mai, 2013 — S. 18
    ISBN: 978-974-672-799-0
    Sprache
    Englisch
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    +49 30 838 62550
    lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    In the last years, novel diagnostic methods have more and more supported or replaced classical detection methods in food safety. Especially DNA sequence based methods are applied. With these methods, a fast detection of e.g. foodborne pathogens or GMO is possible. In addition, a quantification of these agents can be achieved. PCR based methods can be used to further characterize microorganisms in food (detection of virulence factors, antimicrobial resistance markers, stress response).Genotyping enables us to carry out outbreak investigations by comparing bacterial strains from different sources or to characterize bacterial populations in complex habitats.
    Still, some aspects have to be solved:
    - How to distinguish viable from dead bacterial cells esp. in processed food?
    - The role of viable but not culturable bacterial cells;
    - Standardization of genotyping methods to enable a comparison of strains from different regions;
    - Bioinformatical analysis.