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    Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus (2013)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Chai, Weidong
    Burwinkel, Michael
    Wang, Zhenya
    Palissa, Christiane
    Esch, Bettina
    Twardziok, Sven
    Rieger, Juliane
    Wrede, Paul
    Schmidt, Michael F G
    Quelle
    Archives of virology; 158(4) — S. 799–807
    ISSN: 0304-8608
    Sprache
    Englisch
    Verweise
    DOI: 10.1007/s00705-012-1543-0
    Pubmed: 23188495
    Kontakt
    Institut für Veterinär-Anatomie

    Koserstr. 20
    14195 Berlin
    +49 30 838 53555
    anatomie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The enteropathogenic coronavirus transmissible gastroenteritis virus (TGEV) causes severe disease in young piglets. We have studied the protective effects of the probiotic Enterococcus faecium NCIMB 10415 (E. faecium), which is approved as a feed additive in the European Union, against TGEV infection. E. faecium was added to swine testicle (ST) cells before, concomitantly with, or after TGEV infection. Viability assays revealed that E. faecium led to a dose-dependent rescue of viability of TGEV-infected cells reaching nearly to complete protection. Virus yields of the E. faecium-treated cultures were reduced by up to three log10 units. Western blot analysis of purified TGEV revealed that the levels of all viral structural proteins were reduced after E. faecium treatment. Using transmission electron microscopy, we observed attachment of TGEV particles to the surface of E. faecium which might be a means to trap virus and to prevent infection. Increased production of nitric oxide in the cells treated with E. faecium and elevated expression of interleukin 6 and 8 pointed to stimulated cellular defense as a mechanism to fight TGEV infection.