Publikationsdatenbank

Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus (2013)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Chai, Weidong

Burwinkel, Michael

Wang, Zhenya

Palissa, Christiane

Esch, Bettina

Twardziok, Sven

Rieger, Juliane

Wrede, Paul

Schmidt, Michael F G

Quelle

Archives of virology; 158(4) — S. 799–807

ISSN: 0304-8608

Sprache

Englisch

Verweise

DOI: 10.1007/s00705-012-1543-0

Pubmed: 23188495

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 53555
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The enteropathogenic coronavirus transmissible gastroenteritis virus (TGEV) causes severe disease in young piglets. We have studied the protective effects of the probiotic Enterococcus faecium NCIMB 10415 (E. faecium), which is approved as a feed additive in the European Union, against TGEV infection. E. faecium was added to swine testicle (ST) cells before, concomitantly with, or after TGEV infection. Viability assays revealed that E. faecium led to a dose-dependent rescue of viability of TGEV-infected cells reaching nearly to complete protection. Virus yields of the E. faecium-treated cultures were reduced by up to three log10 units. Western blot analysis of purified TGEV revealed that the levels of all viral structural proteins were reduced after E. faecium treatment. Using transmission electron microscopy, we observed attachment of TGEV particles to the surface of E. faecium which might be a means to trap virus and to prevent infection. Increased production of nitric oxide in the cells treated with E. faecium and elevated expression of interleukin 6 and 8 pointed to stimulated cellular defense as a mechanism to fight TGEV infection.