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    Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification (2008)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Guenther, Sebastian
    Groth, Ingrid
    Schierack, Peter
    Grabley, Susanne
    Munder, Thomas
    Quelle
    Journal of basic microbiology : an internat. journal on morphology, physiology, genetics, and ecology of microorganisms; 48(4) — S. 315–318
    ISSN: 0233-111x
    Sprache
    Englisch
    Verweise
    DOI: 10.1002/jobm.200800038
    Pubmed: 18720491
    Kontakt
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    Tel.+49 30 83 8-518 40/518 43 Fax.+49 30 838 45 18 51
    email:mikrobiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co-hybridization with genomic DNA on an oligonuclotide microarray with optical readout.