Publikationsdatenbank

Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification (2008)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Guenther, Sebastian

Groth, Ingrid

Schierack, Peter

Grabley, Susanne

Munder, Thomas

Quelle

Journal of basic microbiology : an internat. journal on morphology, physiology, genetics, and ecology of microorganisms

Bandzählung: 48

Heftzählung: 4

Seiten: 315 – 318

ISSN: 0233-111x

Sprache

Englisch

Verweise

DOI: 10.1002/jobm.200800038

Pubmed: 18720491

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co-hybridization with genomic DNA on an oligonuclotide microarray with optical readout.