Publication Database

Population genetic structure and cladistic analysis of Trypanosoma brucei isolates (2003)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Agbo, E. C.

Clausen, P. H.

Büscher, P.

Majiwa, P. A. O.

Claassen, E.

Pas, MFW te

Quelle

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases ; MEEGID

Bandzählung: 3

Heftzählung: 3

Seiten: 165 – 174

ISSN: 1567-1348

Sprache

Englisch

Verweise

Pubmed: 14522180

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then performed a cladistic analysis to trace relationships and evolution, using stocks and clones recovered from geographically and temporally matched hosts, including inter-strain comparisons with T. b. gambiense stocks and clones. Our results show that while there was close genetic relatedness among parasite populations from the same geographical region, micro-heterogeneities exist between different stocks. Data are presented that indicate that not every human sleeping sickness focus may be associated with a particular human-infective trypanosome strain responsible for long-term stability of the reference focus. We provide evidence of genetic sub-structuring among type 1 T. b. gambiense stocks, which has potentially important implications for molecular epidemiology of T. brucei.