Fachbereich Veterinärmedizin



    Population genetic structure and cladistic analysis of Trypanosoma brucei isolates (2003)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Agbo, E. C.
    Clausen, P. H.
    Büscher, P.
    Majiwa, P. A. O.
    Claassen, E.
    Pas, MFW te
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases; 3(3) — S. 165–174
    ISSN: 1567-1348
    Pubmed: 14522180
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    +49 30 838 62310

    Abstract / Zusammenfassung

    Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then performed a cladistic analysis to trace relationships and evolution, using stocks and clones recovered from geographically and temporally matched hosts, including inter-strain comparisons with T. b. gambiense stocks and clones. Our results show that while there was close genetic relatedness among parasite populations from the same geographical region, micro-heterogeneities exist between different stocks. Data are presented that indicate that not every human sleeping sickness focus may be associated with a particular human-infective trypanosome strain responsible for long-term stability of the reference focus. We provide evidence of genetic sub-structuring among type 1 T. b. gambiense stocks, which has potentially important implications for molecular epidemiology of T. brucei.