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    Effects of toltrazuril and ponazuril on the fine structure and multiplication of tachyzoites of the NC-1 strain of Neospora caninum (a synonym of Hammondia heydorni) in cell cultures (2004)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Darius, A. K.
    Mehlhorn, H.
    Heydorn, A. O.
    Quelle
    Parasitology research; 92(6) — S. 453–458
    ISSN: 0932-0113
    Sprache
    Englisch
    Verweise
    Pubmed: 14963772
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    +49 30 838 62310
    parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Rhesus monkey kidney cell cultures were used to propagate tachyzoites of the NC-1 strain of Neospora caninum/syn.Hammondia heydorni). The infected cell cultures were incubates for 4-12h in media containing 0,1,10 or 100µg/ml of either toltrazuril or ponazuril. The effects were studied by light and electron microscopy. Drug dosages of at least 30µg/ml were needed to eliminate the parasites. Ponazuril was found /with respect to the reduction of the number of parasites) to be less effective at dosages of 30µg/ml compared to toltrazuril. However, the damage to the tachyzoites being incubated in 30µg toltrazuril or ponazuril seen by electron microscopy was so significant that it was surely lethal.The initial damage occurred within the apicoplast and the tubular mitochondrion in all cases, thus destroying two of the most important cell organelles.