Publication Database

Effects of toltrazuril and ponazuril on the fine structure and multiplication of tachyzoites of the NC-1 strain of Neospora caninum (a synonym of Hammondia heydorni) in cell cultures (2004)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Darius, A. K.

Mehlhorn, H.

Heydorn, A. O.

Quelle

Parasitology research

Bandzählung: 92

Heftzählung: 6

Seiten: 453 – 458

ISSN: 0932-0113

Sprache

Englisch

Verweise

Pubmed: 14963772

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Rhesus monkey kidney cell cultures were used to propagate tachyzoites of the NC-1 strain of Neospora caninum/syn.Hammondia heydorni). The infected cell cultures were incubates for 4-12h in media containing 0,1,10 or 100µg/ml of either toltrazuril or ponazuril. The effects were studied by light and electron microscopy. Drug dosages of at least 30µg/ml were needed to eliminate the parasites. Ponazuril was found /with respect to the reduction of the number of parasites) to be less effective at dosages of 30µg/ml compared to toltrazuril. However, the damage to the tachyzoites being incubated in 30µg toltrazuril or ponazuril seen by electron microscopy was so significant that it was surely lethal.The initial damage occurred within the apicoplast and the tubular mitochondrion in all cases, thus destroying two of the most important cell organelles.