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    Measurement of air-borne pyrogens by the in vitro pyrogen test (IPT) based on human whole blodd cytokine response (2003)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Kindiger, I.
    Fennrich, S.
    Zucker, B.
    Linsel, G.
    von Aulock, S.
    Hartung, T.
    Quelle
    European journal of parenteral & pharmaceutical sciences; 8(3) — S. 65–69
    ISSN: 0964-4679
    Sprache
    Englisch
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    Tel.+49 30 8385 1845 Fax.+49 30 83845 1863
    email:tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The impact of environmental micro-organisms as well as their fragments and components, especially endotoxins, on human health is increasingly recognised. Different syndromes have been described in connection with inhaled air-borne microbiological contamination, e.g, sick building syndrome, humidifier lung, organic dust toxic syndrome (ODTS) and "Monday illness" . Air-conditioning systems intensify this problem, as does the collection of biological waste in households, which represents a substantial source of air-born e pollution. In 1995 we described a new method for the detect ion or pyrogenic contamination. This sensitive test (in vitro pyrogen test: IPT ) uses the natural reaction of the immune system to detect a broad spectrum of pyrogens in hum an blond. Safety tests in injectable drugs represent the ma in application and the test has been success fully validated for inclusion into the Euro pea n Pharmacopoeia. Here, the test was adapted to the detection of environmental air-borne pyrogens. Air was drawn through a filter which was then incubated directly with diluted human whole blood . The release of the inflammatory cytokine , interleukin-1β (IL-1β), was measured by ELlSA . ln animal stables, up to 3 x 106 endotoxin equivalent units (EEU) were found per cubic meter of air.