Fachbereich Veterinärmedizin



    Phenotypic and genotypic characterization of Prototheca zopfii in a dog with enteric signs (2009)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Ribeiro, Márcio Garcia
    Rodrigues de Farias, Marconi
    Roesler, Uwe
    Roth, Katja
    Rodigheri, Sabrina Mari
    Ostrowsky, Marco Antonio
    Salerno, Tatiana
    Siqueira, Amanda Keller
    Fernandes, Marta Catarina
    Research in Veterinary Science; 87(3) — S. 479–481
    ISSN: 0034-5288
    DOI: 10.1016/j.rvsc.2009.04.015
    Pubmed: 19520405
    Institut für Tier- und Umwelthygiene

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    14169 Berlin
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    Abstract / Zusammenfassung

    This is a case report of enteric protothecosis caused by Prototheca zopfii in an eight-year-old male mixed breed dog with a history of chronic bloody diarrhea, loss of appetite and weight loss. Algae were isolated from rectal scrapings in defibrinated sheep blood agar and dextrose Sabouraud agar. Cytological evaluation showed the presence of globular and cylindrical organisms with a defined capsule and variable number of endospores, characteristic of the genus Prototheca, in the rectum of the animal. Scanning electron microscopy of P. zopfii strains at different development stages confirmed the diagnosis of algal infection. Molecular identification using a conserved 18S rDNA gene sequence determined that the strain belonged to genotype 2. This report describes success on treatment of canine protothecosis, diagnosed based on clinical, cytological, microbiological, scanning electron microscopy and genotypical findings.