Publikationsdatenbank

Phenotypic and genotypic characterization of Prototheca zopfii in a dog with enteric signs (2009)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ribeiro, Márcio Garcia

Rodrigues de Farias, Marconi

Roesler, Uwe

Roth, Katja

Rodigheri, Sabrina Mari

Ostrowsky, Marco Antonio

Salerno, Tatiana

Siqueira, Amanda Keller

Fernandes, Marta Catarina

Quelle

Research in Veterinary Science

Bandzählung: 87

Heftzählung: 3

Seiten: 479 – 481

ISSN: 0034-5288

Sprache

Englisch

Verweise

DOI: 10.1016/j.rvsc.2009.04.015

Pubmed: 19520405

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

This is a case report of enteric protothecosis caused by Prototheca zopfii in an eight-year-old male mixed breed dog with a history of chronic bloody diarrhea, loss of appetite and weight loss. Algae were isolated from rectal scrapings in defibrinated sheep blood agar and dextrose Sabouraud agar. Cytological evaluation showed the presence of globular and cylindrical organisms with a defined capsule and variable number of endospores, characteristic of the genus Prototheca, in the rectum of the animal. Scanning electron microscopy of P. zopfii strains at different development stages confirmed the diagnosis of algal infection. Molecular identification using a conserved 18S rDNA gene sequence determined that the strain belonged to genotype 2. This report describes success on treatment of canine protothecosis, diagnosed based on clinical, cytological, microbiological, scanning electron microscopy and genotypical findings.