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    Ergebnisse eines deutschlandweiten Ringversuches zur Implementierung der Bouillon-mikrodilution zur Bestimmung der minimalen Hemmkonzentration (MHK) bei Bakterien von Tieren:
    Results from a German interlaboratory test to establish the broth microdilution method for the determination of the minimum inhibitory concentration of bacteria from animals (2005)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Wallmann, Jürgen
    Böttner, Alexander
    Hafez, H Mohamed
    Kehrenberg, Corinna
    Kietzmann, Manfred
    Klarmann, Dieter
    Klein, Günter
    Krabisch, Peter
    Kühn, Tilman
    Luhofer, Gabriele
    Richter, Angelika
    Schwarz, Stefan
    Sigge, Claudia
    Traeder, Wolfgang
    Waldmann, Karl-Heinz
    Werckenthin, Christiane
    Zschiesche, Eva
    Quelle
    Berliner und Münchener tierärztliche Wochenschrift; 118(5-6) — S. 205–213
    ISSN: 0005-9366
    Verweise
    Pubmed: 15918484
    Kontakt
    Institut für Pharmakologie und Toxikologie

    Koserstr. 20
    14195 Berlin
    Tel.+49 30 838 53221 Fax.+49 30 838 53112
    email:pharmakologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    In accordance with NCCLS guideline M31-A2, the DVG working group "antimicrobial resistance" developed a standard operating procedure (SOP) for the determination of the minimal inhibitory concentration (MIC) of antimicrobial agents by broth microdilution. This SOP was evaluated for its fitness for use in a national interlaboratory test. A total of 32 participating laboratories tested five strains (including two internationally accepted reference strains and three field strains representing in total three different bacterial species) three times at a one week interval each, using uniform microtitre plates. In 31 of the 32 laboratories more than 80% of MIC determinations performed yielded values in the expected range. In total 94.0% of the results were reproducible, with a lesser deviation of 4.0% from the expected values for laboratories performing MIC determination as a matter of routine (46.9%), compared to 7.9% for laboratories without such routine (53.1%). Comparing the consistency of results on the basis of the tested strains, a higher reproducibility of the results was observed for reference strains (96.1%) than for field strains (92.6%). In particular results obtained for the Streptococcus uberis field strain were afflicted with a higher error ratio (98 deviations from the expected values). Among the tested antimicrobial agents, a higher variability of results was recorded only for gentamicin with 16.7% divergent MIC determinations (mean value 6.0%). The high reproducibility of the results confirmed by this interlaboratory study underlines the robustness of the developed SOP as well as broth microdilutions as the method of choice for MIC determina tion.