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    Angiogenesis and vasculogenesis in the corpus luteum in vitro (2009)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Kässmeyer, Sabine (WE 1)
    Plendl, Johanna (WE 1)
    Quelle
    Clinical hemorheology and microcirculation; 41(2) — S. 83–101
    ISSN: 1386-0291
    Sprache
    Englisch
    Verweise
    DOI: 10.3233/CH-2009-1159
    Pubmed: 19252232
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    Institut für Veterinär-Anatomie

    Koserstr. 20
    14195 Berlin
    Tel.+49 30 838 53555 Fax.+49 30 838-53480
    email:anatomie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Despite standardized cultivation conditions different morphological forms of vascular development and different angiogenic potency were detected in endothelial cells of the corpus luteum in vitro. The aim of the present study was a comparative investigation of these luteal endothelial cell cultures by light and electron microscopy as well as immunocytochemistry including the use of endothelial and endothelial progenitor markers (CD34, VEGF-R2 and CD31). Screening of cellular proliferation in endothelial cells from regressing corpus luteum (ECreg) showed that vascular development in vitro followed the steps of the angiogenic cascade which starts with a linear alignment of cells, followed by circular side-by-side arrangement. In contrast endothelial cells from developing corpus luteum (ECdev) showed highly active cells and in vitro vascular development was initiated by specific cell groups, so-called "Vascular Initiation Points" (VIP). These cells showed intense proliferation, built typical cellular clusters reminiscent of de novo vasculogenesis and organized into capillary-like tubes with an internal lumen as shown by electron microscopy.