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    In vitro studies to investigate the impact of catecholamines and adrenergic substances on the activity of phagocytes in common carp (Cyprinus carpio) (2006)

    Art
    Poster
    Autoren
    Neumann, N
    Gaete, A
    Knopf, K
    Pietsch, C
    Tönhardt, H
    Kloas, W
    Kongress
    17. Symposium der Fachgruppe Physiologie und Biochemie der DVG e.V.
    Gießen, 19. – 21.02.2006
    Quelle
    Sprache
    Englisch
    Kontakt
    Institut für Veterinär-Physiologie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62600
    physiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Diseases in fish are serious problems in aquaculture. The outbreak of fish disease is largely dependent on environmental and endogenous factors. Recent studies revealed that bidirectional communication between the endocrine and immune system via hormones and cytokines exists at the level of teleost fish. In our work we wanted to ascertain the impact of stress on the activity of the innate immune system in common carp (Cyprinus carpio).
    Phagocytes playing a pivotal role in the defence of microorganisms in fish are isolated from head kidney, kidney and spleen via density centrifugation (Percoll) and incubated on microtiter plates in the presence of catecholamines (norepinephrine, epinephrine and dopamine), adrenergic agonists (phenylephrine, isoproterenol), as well as the ß- adrenergic antagonist propranolol and the corticosteroid cortisol.
    After stimulation of the phagocytes with different concentrations of lipopolysaccharide (LPS) microbicidal activity is evaluated by the detection of nitric oxide (NO) via Griess Reagent.
    Furthermore the production of superoxide anion (O2-) after stimulation of the phagocytes with phorbol myristate acetate (PMA) is measured. During this reaction Nitroblue Tetrazolium (NBT) is reduced by O2- into blue Formazan.
    First results show the modulatory effect of the applied substances on the microbicidal activity of phagocytes in vitro.