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Diseases in fish are serious problems in aquaculture. The outbreak of fish disease is largely dependent on environmental and endogenous factors. Recent studies revealed that bidirectional communication between the endocrine and immune system via hormones and cytokines exists at the level of teleost fish. In our work we wanted to ascertain the impact of stress on the activity of the innate immune system in common carp (Cyprinus carpio).
Phagocytes playing a pivotal role in the defence of microorganisms in fish are isolated from head kidney, kidney and spleen via density centrifugation (Percoll) and incubated on microtiter plates in the presence of catecholamines (norepinephrine, epinephrine and dopamine), adrenergic agonists (phenylephrine, isoproterenol), as well as the ß- adrenergic antagonist propranolol and the corticosteroid cortisol.
After stimulation of the phagocytes with different concentrations of lipopolysaccharide (LPS) microbicidal activity is evaluated by the detection of nitric oxide (NO) via Griess Reagent.
Furthermore the production of superoxide anion (O2-) after stimulation of the phagocytes with phorbol myristate acetate (PMA) is measured. During this reaction Nitroblue Tetrazolium (NBT) is reduced by O2- into blue Formazan.
First results show the modulatory effect of the applied substances on the microbicidal activity of phagocytes in vitro.