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    Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli (2010)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Moriel, Danilo Gomes
    Bertoldi, Isabella
    Spagnuolo, Angela
    Marchi, Sara
    Rosini, Roberto
    Nesta, Barbara
    Pastorello, Ilaria
    Corea, Vanja A Mariani
    Torricelli, Giulia
    Cartocci, Elena
    Savino, Silvana
    Scarselli, Maria
    Dobrindt, Ulrich
    Hacker, Jörg
    Tettelin, Hervé
    Tallon, Luke J
    Sullivan, Steven
    Wieler, Lothar H
    Ewers, Christa
    Pickard, Derek
    Dougan, Gordon
    Fontana, Maria Rita
    Rappuoli, Rino
    Pizza, Mariagrazia
    Serino, Laura
    Quelle
    Proceedings of the National Academy of Sciences of the United States of America; 107(20) — S. 9072–9077
    ISSN: 0027-8424
    Sprache
    Englisch
    Verweise
    DOI: 10.1073/pnas.0915077107
    Pubmed: 20439758
    Kontakt
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843
    mikrobiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance of these bacteria. We have determined the genome sequence of ExPEC IHE3034 (ST95) isolated from a case of neonatal meningitis and compared this to available genome sequences of other ExPEC strains and a few nonpathogenic E. coli. We found 19 genomic islands present in the genome of IHE3034, which are absent in the nonpathogenic E. coli isolates. By using subtractive reverse vaccinology we identified 230 antigens present in ExPEC but absent (or present with low similarity) in nonpathogenic strains. Nine antigens were protective in a mouse challenge model. Some of them were also present in other pathogenic non-ExPEC strains, suggesting that a broadly protective E. coli vaccine may be possible. The gene encoding the most protective antigen was detected in most of the E. coli isolates, highly conserved in sequence and found to be exported by a type II secretion system which seems to be nonfunctional in nonpathogenic strains.