Publikationsdatenbank

Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli (2010)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Moriel, Danilo Gomes

Bertoldi, Isabella

Spagnuolo, Angela

Marchi, Sara

Rosini, Roberto

Nesta, Barbara

Pastorello, Ilaria

Corea, Vanja A Mariani

Torricelli, Giulia

Cartocci, Elena

Savino, Silvana

Scarselli, Maria

Dobrindt, Ulrich

Hacker, Jörg

Tettelin, Hervé

Tallon, Luke J

Sullivan, Steven

Wieler, Lothar H

Ewers, Christa

Pickard, Derek

Dougan, Gordon

Fontana, Maria Rita

Rappuoli, Rino

Pizza, Mariagrazia

Serino, Laura

Quelle

Proceedings of the National Academy of Sciences of the United States of America

Bandzählung: 107

Heftzählung: 20

Seiten: 9072 – 9077

ISSN: 1091-6490

Sprache

Englisch

Verweise

DOI: 10.1073/pnas.0915077107

Pubmed: 20439758

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance of these bacteria. We have determined the genome sequence of ExPEC IHE3034 (ST95) isolated from a case of neonatal meningitis and compared this to available genome sequences of other ExPEC strains and a few nonpathogenic E. coli. We found 19 genomic islands present in the genome of IHE3034, which are absent in the nonpathogenic E. coli isolates. By using subtractive reverse vaccinology we identified 230 antigens present in ExPEC but absent (or present with low similarity) in nonpathogenic strains. Nine antigens were protective in a mouse challenge model. Some of them were also present in other pathogenic non-ExPEC strains, suggesting that a broadly protective E. coli vaccine may be possible. The gene encoding the most protective antigen was detected in most of the E. coli isolates, highly conserved in sequence and found to be exported by a type II secretion system which seems to be nonfunctional in nonpathogenic strains.