Fachbereich Veterinärmedizin



    Closely related Campylobacter jejuni strains from different sources reveal a generalist rather than a specialist lifestyle (2011)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Gripp, Eugenia
    Hlahla, Daniela
    Didelot, Xavier
    Kops, Friederike
    Maurischat, Sven
    Tedin, Karsten
    Alter, Thomas
    Ellerbroek, Lüppo
    Schreiber, Kerstin
    Schomburg, Dietmar
    Janssen, Traute
    Bartholomäus, Patrick
    Hofreuter, Dirk
    Woltemate, Sabrina
    Uhr, Markus
    Brenneke, Birgit
    Grüning, Petra
    Gerlach, Gerald
    Wieler, Lothar
    Suerbaum, Sebastian
    Josenhans, Christine
    BMC genomics; 12 — S. 584
    ISSN: 1471-2164
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000017240
    DOI: 10.1186/1471-2164-12-584
    Pubmed: 22122991
    Institut für Mikrobiologie und Tierseuchen

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    Abstract / Zusammenfassung

    Campylobacter jejuni and Campylobacter coli are human intestinal pathogens of global importance. Zoonotic transmission from livestock animals or animal-derived food is the likely cause for most of these infections. However, little is known about their general and host-specific mechanisms of colonization, or virulence and pathogenicity factors. In certain hosts, Campylobacter species colonize persistently and do not cause disease, while they cause acute intestinal disease in humans.

    Here, we investigate putative host-specificity using phenotypic characterization and genome-wide analysis of genetically closely related C. jejuni strains from different sources. A collection of 473 fresh Campylobacter isolates from Germany was assembled between 2006 and 2010 and characterized using MLST. A subset of closely related C. jejuni strains of the highly prevalent sequence type ST-21 was selected from different hosts and isolation sources. PCR typing of strain-variable genes provided evidence that some genes differed between these strains. Furthermore, phenotypic variation of these strains was tested using the following criteria: metabolic variation, protein expression patterns, and eukaryotic cell interaction. The results demonstrated remarkable phenotypic diversity within the ST-21 group, which however did not correlate with isolation source. Whole genome sequencing was performed for five ST-21 strains from chicken, human, bovine, and food sources, in order to gain insight into ST-21 genome diversity. The comparisons showed extensive genomic diversity, primarily due to recombination and gain of phage-related genes. By contrast, no genomic features associated with isolation source or host were identified.

    The genome information and phenotypic data obtained in vitro and in a chicken infection model provided little evidence of fixed adaptation to a specific host. Instead, the dominant C. jejuni ST-21 appeared to be characterized by phenotypic flexibility and high genetic microdiversity, revealing properties of a generalist. High genetic flexibility might allow generalist variants of C. jejuni to reversibly express diverse fitness factors in changing environments.