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    Identification and phenotyping of leukocytes in bovine bronchoalveolar lavage fluid. (2004)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Soethout, EC
    Mueller, K E
    van der Belt, A J
    Rutten, V. P.
    Quelle
    Clinical and diagnostic laboratory immunology; 11(4) — S. 795–798
    ISSN: 1071-412x
    Sprache
    Englisch
    Verweise
    Pubmed: 15242961
    Kontakt
    Klinik für Klauentiere

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    Gebäude 26
    14163 Berlin
    Tel.+49 30 838 62261 Fax.+49 30 838 62512
    email:klauentierklinik@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    A method is proposed to identify leukocyte subpopulations in bovine bronchoalveolar lavage fluid by dual-laser flow cytometry. The technique uses several parameters, i.e., exclusion of highly autofluorescent alveolar macrophages and inclusion of leukocytes on the basis of labeling by specific antibodies and light scatter characteristics.