Publication Database

Detection of chimpanzee polyomavirus-specific antibodies in captive and wild-caught chimpanzees using yeast-expressed virus-like particles (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Zielonka, Anja

Verschoor, Ernst J.

Gedvilaite, Alma

Roesler, Uwe

Müller, Hermann

Johne, Reimar

Quelle

Virus research : an international journal of molecular and cellular virology

Bandzählung: 155

Heftzählung: 2

Seiten: 514 – 519

ISSN: 0168-1702

Sprache

Englisch

Verweise

DOI: 10.1016/j.virusres.2010.12.009

Pubmed: 21187117

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Chimpanzee polyomavirus (ChPyV) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum PCR. Its pathogenicity and the distribution among chimpanzees are unknown so far. Here, the major capsid protein VP1 of ChPyV was expressed in yeast cells. Virus-like particles (VLPs) with a diameter of approximately 45nm were demonstrated although the efficiency of VLP formation was low as compared to monkey polyomavirus SV40-VLPs. The ChPyV-VLP preparation did not agglutinate human erythrocytes. Low cross-reactions between ChPyV- and SV40-VLP-specific sera were detected by immunoblotting, but not by ELISA. Testing of 163 sera derived from captive and wild-caught healthy chimpanzees using an ELISA based on the ChPyV-VLPs resulted in 11.7% positive results, ranging from 0% to 56% in different groups. The VLPs may be used in future to assess the distribution of ChPyV infections among other animal species and humans.