Fachbereich Veterinärmedizin



    Genotyping of bovine Prototheca mastitis isolates from Poland (2011)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Jagielski, Tomasz
    Lassa, Henryka
    Ahrholdt, Jennifer
    Malinowski, Edward
    Roesler, Uwe
    Veterinary Microbiology; 149(1/2) — S. 283–287
    ISSN: 0378-1135
    DOI: 10.1016/j.vetmic.2010.09.034
    Pubmed: 21055886
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845

    Abstract / Zusammenfassung

    Bovine mastitis due to unicellular, achlorophyllous algae of the genus Prototheca is a serious and complex ailment that accounts for high economic losses in the dairy industry. Bovine protothecal mastitis has been almost exclusively associated with only one species Prototheca zopfii. Recently, based on the 18S rDNA sequence analysis, P. zopfii has been divided into three genotypes (1-3), of which P. zopfii genotype 3 has been given the status of a new species Prototheca blaschkeae sp. nov. The aim of this study was to investigate the genotypic composition of the population of P. zopfii bovine mastitis isolates from Poland, according to that threefold classification. Of the 44 isolates tested, 43 (98%) were identified as P. zopfii genotype 2. Only one isolate was assigned to the newly established P. blaschkeae species. These findings clearly show the predominance of the P. zopfii genotype 2 in the etiology of bovine mammary protothecosis in Poland, but also underline the involvement of P. blaschkeae in the disease. The study is the first to provide molecular characterization of Polish P. zopfii mastitis isolates, collected from different regions of Poland.