Fachbereich Veterinärmedizin



    Comparing validation of four ELISA-systems for detection of Salmonella derby- and Salmonella infantis-infected pigs (2011)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Roesler, Uwe
    Szabo, Istvan
    Matthies, Claudia
    Albrecht, Kerstin
    Leffler, Martin
    Scherer, Kathrin
    Nöckler, Karsten
    Lehmann, Jörg
    Methner, Ulrich
    Hensel, Andreas
    Truyen, Uwe
    Berliner und Münchener tierärztliche Wochenschrift; 124(7/8) — S. 265–271
    ISSN: 0005-9366
    Pubmed: 21848033
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    Tel.+49 30 8385 1845 Fax.+49 30 83845 1863

    Abstract / Zusammenfassung

    The objective of this study was the comparative evaluation of four indirect Salmonella ELISA tests at study time approved in Germany to detect Salmonella infection in pigs.Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella-specific IgM-, IgA-, and IgG- antibodies. In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs. Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derby-infected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates.