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    Multicentre Etest evaluation of in vitro activity of conventional antifungal drugs against European bovine mastitis Prototheca spp. isolates (2012)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Jagielski, Tomasz
    Buzzini, Pietro
    Lassa, Henryka
    Malinowski, Edward
    Branda, Eva
    Turchetti, Benedetta
    Polleichtner, Angela
    Roesler, Uwe
    Lagneau, Paul-Emile
    Marques, Sara
    Silva, Eliane
    Thompson, Gertrude
    Stachowiak, Radosław
    Bielecki, Jacek
    Forschungsprojekt
    Pathogene Pflanzen: Epidemiologie und Virulenzmerkmale von Prototheken humaner und tierischer Herkunft
    Quelle
    The Journal of antimicrobial chemotherapy; 67(8) — S. 1945–1947
    ISSN: 0305-7453
    Sprache
    Englisch
    Verweise
    DOI: 10.1093/jac/dks134
    Pubmed: 22523316
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    Tel.+49 30 8385 1845 Fax.+49 30 83845 1863
    email:tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Bovine mammary protothecosis is a serious pathology that entails high economic losses in the dairy industry. The disease, the frequency of which has recently been increasing worldwide, is caused by unicellular, achlorophyllous, yeast-like algae of two species: Prototheca zopfii and Prototheca blaschkeae. The objective of this study was to investigate the in vitro activity of a panel of conventional antifungal drugs against Prototheca spp. isolates.

    A total of 144 P. zopfii genotype 2 and P. blaschkeae strains isolated from milk of mastitic cows were subjected to drug susceptibility testing by Etest methodology.

    Five out of ten antifungal drugs tested exhibited no activity against Prototheca spp. isolates. The best activity against Prototheca spp. was demonstrated by amphotericin B (MIC₉₀ of 1.5 mg/L). The MICs differed significantly (P < 0.01) between P. zopfii genotype 2 and P. blaschkeae, with the latter species being more susceptible to amphotericin B and azoles. Marked differences (P < 0.05) in azole and amphotericin B activities were noted among Prototheca spp. isolates originating from different European countries. Based on the correlation coefficients, a considerable cross-interaction was found among MICs of azoles and between MICs of azoles and amphotericin B for Prototheca spp. (P < 0.03).

    This study represents the largest, cross-European evaluation of antifungal activity against Prototheca spp. to date. The activity of amphotericin B against Prototheca spp. validates its potential use as a therapeutic agent against bovine protothecosis. For laboratory testing of drug activity against Prototheca spp., the Etest method is encouraged, due to its technical simplicity, rapidity and high intra- and inter-laboratory reproducibility.