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    Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR (2008)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Erol, I
    Goncuoglu, M
    Ayaz, N D
    Bilir Ormanci, F S
    Hildebrandt, G
    Quelle
    Letters in Applied Microbiology; 47(1) — S. 31–34
    ISSN: 0266-8254
    Sprache
    Englisch
    Verweise
    DOI: 10.1111/j.1472-765X.2008.02379.x
    Pubmed: 18554263
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029
    email:lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing.

    For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. perfringens isolates were found to carry the cpa, gene but in none of the isolates cpb, etx, iap or cpe genes were detected. Results showed that all isolates represented type A and were cpe negative.

    Our results indicate that Cl. perfringens type A is the most common type in turkey meat. Also multiplex PCR is effective and rapid method for typing of Cl. perfringens.

    It is the first study about molecular typing of Cl. perfringens using multiplex PCR in turkey meat samples in Turkey.